ASB-16 is a useful zwitterionic detergent for the solubilization of proteins including previously undetected membrane proteins. ASB-16 exhibits better protein solubilization properties than CHAPS. Also useful for solubilizing proteins for 2D-gel electrophoresis.
Brij™ 35 is a nonionic polyoxyethylene surfactant that is most frequently used as a component of cell lysis buffers or a surfactant in various HPLC applications.
Cholic acid sodium is the sodium salt of the naturally occurring bile acid Cholic acid. Acts as a non-denaturing ionic detergent used for extraction of membrane proteins.
DDMAB is a water-soluble zwitterionic detergent useful for the extraction of mycoplasma membrane antigens under mild conditions.
Sodium deoxycholate is a bile salt and an ionic detergent. It has been used in the lysis buffer prepared for brown adipose tissue. It has also been used in the decellularization of aortic valvular conduit.
A highly purified form of deoxycholic acid sodium (Cat. No. AUR10320). Useful as a detergent to solubilize membrane-bound proteins in their native state.
HEPES is a zwitterionic N-substituted aminosulfonic acid buffer (Absorbance: <0.10 at 260 nm, 1.0 mM, H2O). Active in the pH range of 6.0 to 8.5. Has a pKa of 7.6 at 20°C.
IGEPAL® CA-630 is a nonionic, non-denaturing detergent suitable for solubilization, isolation and purification of membrane protein complexes. It is chemically indistinguishable from Nonidet P-40.
NDSB-195 is a non-detergent sulfobetaine that has been reported to prevent protein aggregation and facilitate the renaturation of chemically and thermally denatured proteins. Zwitterionic over a wide pH range. Easily removed by dialysis
NDSB-211, is a nondetergent-sulfobetain. It is zwitterionic over a wide pH range, easily removed by dialysis and shows no significant absorption in the near UV range.
NDSB-221 is a nondetergent sulfobetaine that has been found to improve unfolding reversibility. Increases the extraction yield of membrane, nuclear and cytoskeletal associated proteins.
A non-ionic surfactant, used to solubilize cerebral GABA receptors and for solubilization of proteins and lipids of the scallop gill ciliary membrane and of membranes from breast cancer tumors.
